Nikon N-STORM microscope
Here at COMPARE depending on the type of sample you wish to image you can use Direct Stochastic Optical Reconstruction Microscopy (dSTORM) or Photoactivatable Localisation Microscopy (PALM).
The Nikon N-STORM with perfect focus and temperature control is available at The University of Birmingham site, for all enquiries please contact D.M.Kavanagh@bham.ac.uk
Modalities |
dSTORM, PALM (TIRF and 3D), TIRFM and DIC |
Objectives |
100x TIRF oil lens, 1.49 NA, 0.12 mm WD 60x TIRF oil lens, 1.49 NA, 0.12 mm WD 40x oil lens, 0.95 NA, 0.25-0.16 mm WD 20x oil lens, 0.75 NA, 1 mm WD |
Sample platform |
35 mm glass bottom dishes (e.g. Matek), AttoflourTM chamber, dimple mounted slides |
Lamp excitation source |
Mercury Lamp – Visible to Near IR |
Laser lines |
405, 488, 561 and 640 nm |
Chroma filter sets |
49000-ET-DAPI 49002-ET-EGFP (FITC/CY2) 49008-ET mCherry, Texas Red 49006-ET CY5 TRF49904-ET-488 Laser Bandpass Set TRF49909-ET-561 Laser Bandpass Set TRF49913-ET-633-640 Laser Longpass Set TRF59904-ET-488/561 Laser Dual Band Set |
Camera |
Andor iXon Ultra EMCCD camera 512 x 512 56 fps, 256 x256 102 fps, 128 x128 212 fps |
Environmental control |
Okolab temperature and humidity control incubator |
Software |
NIS elements and FIJI |
Operating system |
Windows 7 |
Data transfer |
1 GB network connection - no external hard drives allowed |
Usage |
Staff-assisted and self-use allowed (following training) |