University of Nottingham
  

Nikon N-STORM microscope

Here at COMPARE depending on the type of sample you wish to image you can use Direct Stochastic Optical Reconstruction Microscopy (dSTORM) or Photoactivatable Localisation Microscopy (PALM).

The Nikon N-STORM with perfect focus and temperature control is available at The University of Birmingham site, for all enquiries please contact D.M.Kavanagh@bham.ac.uk

Modalities  dSTORM, PALM (TIRF and 3D), TIRFM and DIC
Objectives 100x TIRF oil lens, 1.49 NA, 0.12 mm WD
60x TIRF oil lens, 1.49 NA, 0.12 mm WD
40x oil lens, 0.95 NA, 0.25-0.16 mm WD
20x oil lens, 0.75 NA, 1 mm WD
Sample platform 35 mm glass bottom dishes (e.g. Matek), AttoflourTM chamber, dimple mounted slides
Lamp excitation source Mercury Lamp – Visible to Near IR
Laser lines 405, 488, 561 and 640 nm 
Chroma filter sets 49000-ET-DAPI
49002-ET-EGFP (FITC/CY2)
49008-ET mCherry, Texas Red
49006-ET CY5
TRF49904-ET-488 Laser Bandpass Set
TRF49909-ET-561 Laser Bandpass Set
TRF49913-ET-633-640 Laser Longpass Set
TRF59904-ET-488/561 Laser Dual Band Set
Camera Andor iXon Ultra EMCCD camera 
512 x 512 56 fps, 256 x256 102 fps, 128 x128 212 fps
Environmental control Okolab temperature and humidity control incubator
Software NIS elements and FIJI
Operating system Windows 7
Data transfer 1 GB network connection - no external hard drives allowed
Usage  Staff-assisted and self-use allowed (following training)

 


 

 

 

In Partnership: The Universities of Birmingham and Nottingham


University of Birmingham
Edgbaston,
Birmingham
B15 2TT

Telephone: +44 (0)121 414 3974
info@birmingham-nottingham.ac.uk

The University of Nottingham
University Park,
Nottingham
NG7 2RD

Telephone: +44 (0)115 846 7554
info@birmingham-nottingham.ac.uk