| Location |
Imaging Suite, WXG92 |
| Imaging Modalities |
3D SIM, 2D SIM, TIRF SIM, H-TIRF |
| Usage |
Assisted and independent use |
| Sample Mounting |
Cover glass 1.5HT (high tolerance ± 5 μm) mounted on slides or 35 mm dishes. |
| Objectives |
100x (NA 1.49) oil
100x (NA 1.35) silicone |
| Laser Lines |
405nm, 488nm, 561nm, 640nm |
| Emission filters |
Chroma ET525/50m (EGFP/Alexa488)
Chroma ET595/50m (mCherry/Alexa561)
Chroma ET 700/75m (Alexa 647/Cy5) |
| Camera |
2x Hamamatsu Flash4.0 sCMOS |
| Imaging splitter |
Cairn TwinCam |
| Emission filters (Twin) |
Gr/Red, Red/Far Red & Gr/Far red |
| Environmental Control |
Full CO2, temperature & humidity |
| PC Operating system |
Windows 10 |
| Acquisition Software |
NIKON NIS Elements 5 |
| Analysis |
NIS Elements 5 workstation, WXG922414 |