Marianas diSPIM microscope
The Marianas light-sheet microscope at COMPARE, offers a single- (iSPIM) or dual- (diSPIM) sided light-sheet arrangement on an inverted microscope with a tilted lens configuration. Application of the dual arrangement results in the collection of two 3D datasets which can be computationally merged together to give 3D isotropic resolution (330 nm in x-y-z). Samples are prepared on conventional glass coverslips and the diSPIM can be used to image cells, cleared tissues (aqueous based clearing solutions only) and small organisms.
Set-up of the COMPARE Marianas diSPIM in The Imaging Suite.
The Marianas light-sheet with custom built OKO environment chamber is available in The University of Birmingham Imaging Suite, for all enquiries please contact D.M.Kavanagh@bham.ac.uk
|| Single and dual inverted SPIM
|SPIM x-y-z resolution
|| 370 x 370 x 760 nm
|diSPIM x-y-z resolution
|| 330 x 330 x 330 nm
|| SPIM: Twin Nikon CFI NIR Apo 40X 0.8 NA 3.5 mm WD (water dipping objectives)
|| Zeiss 40x, 20x and 10x Oil lenses
|| Rectangular coverslips (1.5#, 25 x 50 mm) or petri dishes
|| 488, 561 and 640 nm laser lines
|| Shemrock 440/521/607/700 BrightLine quad bandpass filter
|| Twin Hamamatsu ORCA-Flash4.0 V3 sCMOS
|| Hamamatsu ORCA-ER
|| diSPIM custom designed OKO incubator with temperature, CO2 and humidity control
|| Slidebook 6
|| Windows 7
|| 10 GB network connection - no external hard drives allowed
|| Staff-assisted and self-use allowed (following training)
Switching between illumination and detection on the Marianas diSPIM system.