University of Nottingham
  

Lattice Light Sheet Microscope

Developed by Dr. Eric Betzig and colleagues at the Janelia Research Campus, the Lattice LightSheet Microscope (LLSM) at COMPARE, is one of the first of its kind in the U.K.

LLSM allows non-destructive imaging of living samples in three dimensions over time with unparalleled spatiotemporal resolution. Using a combination of newly developed optical components, the microscope can generate an ultrathin (typically < 600 nm) sheet of light comprised of a non-diffracting 2D optical lattice. A 3D image is generated by scanning the light-sheet through the sample plane by plane, reaching a frame rate of near 300 slices per second. The LLSM is well suited for imaging intracellular dynamics in 4D.

Time lapse movie of actin (green) and tubulin (red) dynamics during proplatelet formation.
Movie courtesy of Malou Zuidscherwoude, University of Birmingham

The Lattice Light Sheet is available at The University of Birmingham site, for all enquiries please contact Joao Correia

Modalities  Lattice light sheet
x-y-z resolution  230 x 230 x 370 nm (dithered mode)
Excitation objective Special Optics 0.65 NA, 3.74 mm WD (water dipping)
Imaging objective  Nikon CFI Apo LWD 25x, 1.1 NA, 3 mm WD (water dipping)
Finder objective Olympus Oil Lens 20x, 0.45 NA
Sample platform 5 mm coverslips 
Excitation sources 405, 488, 560 and 640 nm laser lines
Camera Hamamatsu Orca Flash 4.0 sCMOS
Environmental control Temperature controlled z-stage
Software Slidebook 6
Operating system Windows 7
Data transfer 10 GB network connection - no external hard drives allowed
Usage Only staff-assisted usage (not for self-use)

  

Light sheet cell image

 

 

 

 

 

 

 

Light-sheet profile generated from a Bessel beam illumination source. 
Image courtesy of Dee Kavanagh, University of Birmingham

 

 

 

In Partnership: The Universities of Birmingham and Nottingham


University of Birmingham
Edgbaston,
Birmingham
B15 2TT

Telephone: +44 (0)121 414 3974
info@birmingham-nottingham.ac.uk

The University of Nottingham
University Park,
Nottingham
NG7 2RD

Telephone: +44 (0)115 846 7554
info@birmingham-nottingham.ac.uk