Lattice LightSheet Microscope
Developed by Dr. Eric Betzig and colleagues at the Janelia Research Campus, the Lattice LightSheet Microscope (LLSM) at COMPARE, is one of the first of its kind in the U.K.
LLSM allows non-destructive imaging of living samples in three dimensions over time with unparalleled spatiotemporal resolution. Using a combination of newly developed optical components, the microscope can generate an ultrathin (typically < 600 nm) sheet of light comprised of a non-diffracting 2D optical lattice. A 3D image is generated by scanning the light-sheet through the sample plane by plane, reaching a frame rate of near 300 slices per second. The LLSM is well suited for imaging intracellular dynamics in 4D.
Time lapse movie of actin (green) and tubulin (red) dynamics during proplatelet formation.
Movie courtesy of Malou Zuidscherwoude, University of Birmingham
The Lattice LightSheet is available at The University of Birmingham site, for all enquiries please contact D.M.Kavanagh@bham.ac.uk
||230 x 230 x 370 nm (dithered mode)
||Special Optics 0.65 NA, 3.74 mm WD (water dipping)
||Nikon CFI Apo LWD 25x, 1.1 NA, 3 mm WD (water dipping)
||Olympus Oil Lens 20x, 0.45 NA
||5 mm coverslips
||405, 488, 560 and 640 nm laser lines
||Hamamatsu Orca Flash 4.0 sCMOS
||Temperature controlled z-stage
||10 GB network connection - no external hard drives allowed
||Only staff-assisted usage (not for self-use)
Light-sheet profile generated from a Bessel beam illumination source.
Image courtesy of Dee Kavanagh, University of Birmingham