Date(s)
Thursday 7th November 2024 (12:00-13:00)
Contact
Laura Kilpatrick or Joelle Goulding
Description

Seminar by Dr Laura Wiggins, University of Sheffield on 7th November at 12:00 in E1, Medical School, Queens Medical Centre, University of Nottingham.

Presentation on: No labels, just discoveries: the power of ptychography for single-cell characterisation

With phenotypic heterogeneity in whole cell populations widely recognised, the demand for quantitative and temporal analysis approaches to characterise single-cell morphology and dynamics has increased. To address this, our team developed CellPhe, a pattern recognition toolkit for the unbiased characterisation of cellular phenotypes within time-lapse videos. CellPhe has demonstrated its value in quantifying single-cell responses to drug treatment, facilitating imaging of co-cultures and identifying cell-cell signalling events. However, the complexities of biological data sets and evolution of new imaging technologies call for even more advanced analysis solutions. Here we present CellPhe 2, which builds upon our original cell phenotyping toolkit by incorporating new analysis capabilities for population-level modelling, tracklet analysis and interoperability with existing open-source cell segmentation and tracking software. CellPhe 2 is designed to facilitate analysis of complex data sets from multi-channel fluorescence images through to densely populated samples where conventional segmentation approaches underperform.