Single Molecule Microscopy
Single Molecule Localisation Microscopy (SMLM)
Single Molecule Localisation Microscopy (SMLM) techniques provide invaluable information about the organisation of single molecules within a fixed cellular structure. They employ photoactivatable or photoswitchable fluorophores to localise single molecules with nanometer precision. In contrast to conventional confocal or widefield based imaging techniques where all fluorophores within your imaging plane are viewed at once, in SMLM only a small subset of fluorophores are activated at a time allowing their precise position to be recorded with a camera. Following acquisition, image reconstruction methods are employed to create a high precision map of molecule positions.
SMLM can be performed on our Nikon N-STORM system at the University of Birmingham site.