Marianas diSPIM microscope
The Marianas light-sheet microscope at COMPARE, offers a single- (iSPIM) or dual- (diSPIM) sided light-sheet arrangement on an inverted microscope with a tilted lens configuration. Application of the dual arrangement results in the collection of two 3D datasets which can be computationally merged together to give 3D isotropic resolution (330 nm in x-y-z). Samples are prepared on conventional glass coverslips and the diSPIM can be used to image cells, cleared tissues (aqueous based clearing solutions only) and small organisms.
Set-up of the COMPARE Marianas diSPIM in The Imaging Suite.
The Marianas light-sheet with custom built OKO environment chamber is available in The University of Birmingham Imaging Suite, for all enquiries please contact Joao Correia.
| Modalities |
Single and dual inverted SPIM |
| SPIM x-y-z resolution |
370 x 370 x 760 nm |
| diSPIM x-y-z resolution |
330 x 330 x 330 nm |
| SPIM objectives |
SPIM: Twin Nikon CFI NIR Apo 40X 0.8 NA 3.5 mm WD (water dipping objectives) |
| Finder objectives |
Zeiss 40x, 20x and 10x Oil lenses |
| Sample platform |
Rectangular coverslips (1.5#, 25 x 50 mm) or petri dishes |
| Excitation sources |
488, 561 and 640 nm laser lines |
| Emission filters |
Shemrock 440/521/607/700 BrightLine quad bandpass filter |
| Light-sheet camera |
Twin Hamamatsu ORCA-Flash4.0 V3 sCMOS |
| Finder camera |
Hamamatsu ORCA-ER |
| Environmental control |
diSPIM custom designed OKO incubator with temperature, CO2 and humidity control |
| Software |
Slidebook 6 |
| Operating system |
Windows 7 |
| Data transfer |
10 GB network connection - no external hard drives allowed |
| Usage |
Staff-assisted and self-use allowed (following training) |
Switching between illumination and detection on the Marianas diSPIM system.